zacharalab.johnshopkins.edu
Archived News
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Dr Zachara's Faculty Page. Dr Zachara's Faculty Page. Characterizing a novel cytoprotective pathway. Who has accepted an offer to complete her Degree in Medicine at UCSF (May, 2014). Russell, Albert and Roger. Characterization of the Specificity of O-GlcNAc Reactive Antibodies Under Conditions of Starvation and Stress” has been accepted at Analytical Biochemistry (April, 2014). Marissa Martinez Ph.D. Who was awarded a travel fellowship to the ASBMB. (February, 2014). Kamau Fahie Ph.D. Kamau Fahie Ph.D.
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Lab Resources
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Dr Zachara's Faculty Page. Dr Zachara's Faculty Page. Characterizing a novel cytoprotective pathway. Inducible OGT Null Cells. We have generated a cell line in which OGT can be deleted upon addition of 4-hydroxytamoxifen ( Kazemi et al., 2010. We are happy to share this cell line and provide a detailed protocol for using these cells. Please accompany your request with the following MTA questionnaire, which can be downloaded here. Antibodies used to detect O-GlcNAc. Current Protocols in Protein Science,.
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Protocols
http://zacharalab.johnshopkins.edu/styled-4/downloads/index.html
Dr Zachara's Faculty Page. Dr Zachara's Faculty Page. Characterizing a novel cytoprotective pathway. The following files contain protocols commonly used in the Zachara Lab. Nuclear and Cytoplasmic Fractionation from Frozen Cells. Total Cell Lysates (NP-40). Total Cell Lysates (RIPA). Protein Estimation using the Pierce 660 Reagent. Protein Estimation using the BCA Reagent. Desalting proteins or cell extract with PD10 columns. Desalting proteins with Sephadex G50 (Old School). 96 Well: Counting Cells.
zacharalab.johnshopkins.edu
News and Publications
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Dr Zachara's Faculty Page. Dr Zachara's Faculty Page. Characterizing a novel cytoprotective pathway. Marissa, Peter and Thiago. Whose review “. Stress-Induced O-GlcNAcylation, an Adaptive Process of Injured Cells” i. S in press in Biochemical Society Transactions (. Who have won Travel Awards to present their work at the Society for Glycobiology Annual Meeting (November, 2016). Congratulations to Albert, whose paper. Is in press at the Journal of Proteome Research (September 2016). Chemical genetic tools...
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The Team
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Dr Zachara's Faculty Page. Dr Zachara's Faculty Page. Characterizing a novel cytoprotective pathway. From left to right:. Peter Natov (undergraduate researcher), Jen Groves (Grad. Student), Kamau Fahie Ph.D., Cathrine McKen (Lab Manager), Thiago Dias M.D. (Visiting fellow), Marissa Martinez Ph.D., and Roger Henry (Super Tech.). Not pictured: Austin Maduka. Thiago Braido Dias, M.D.,. Thiago.b.dias@gmail.com. Kamau Fahie, Ph.D.,. In 2011. She is currently a Biological Chemistry Graduate student and is ...
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Common Lab Recipes
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Dr Zachara's Faculty Page. Dr Zachara's Faculty Page. Characterizing a novel cytoprotective pathway. 4-hydroxytamoxifen (500uM): typically this is a 1000x stock. 5mg 4-hydroxytamoxifen (Sigma H7904, MW=387.5). Dissolve in 25mls of ethanol (200 proof). Aliquot in glass vials. Store at -20oC in the dark at -20oC. PIC2 (Protease inhibitor cocktail 2; Calbiochem #539132). Reconstitute each vial of lyophilized protease inhibitor cocktail with 1 ml of DMSO first. Then add 4 ml of water. Whose review “. 2011 Na...
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Favorite Recipes
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Dr Zachara's Faculty Page. Dr Zachara's Faculty Page. Characterizing a novel cytoprotective pathway. The labs favorite chocolate cake. Roger's Favorite Granola Bars. Marissa, Peter and Thiago. Whose review “. Stress-Induced O-GlcNAcylation, an Adaptive Process of Injured Cells” i. S in press in Biochemical Society Transactions (. Austin has been awarded the 2016. Marion B. Sewer Distinguished Scholarship. By the American Society for Biochemistry and Molecular Biology (ASBMB). 725 N Wolfe Street.
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Home
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Dr Zachara's Faculty Page. Dr Zachara's Faculty Page. Characterizing a novel cytoprotective pathway. More than 3000 cytoplasmic,. O-GlcNAc is added and removed by just two enzymes, the O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA). We demonstrated that O-GlcNAcylation of intracellular proteins was a novel regulator of cell survival and a modulator of the cellular stress response. In response to diverse forms of cellular stress, the O-GlcNAc modification is increased on. Elevation of O-GlcNAc levels mo...
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Beyond the bench
http://zacharalab.johnshopkins.edu/styled-5/index.html
Dr Zachara's Faculty Page. Dr Zachara's Faculty Page. Characterizing a novel cytoprotective pathway. Kamau will be performing in Romeo and Juliet. A presentation put on at the Baltimore Performance Kitchen. The season runs from June 20th-30th. For more information and to reserve your free ticket, follow this. Congratulations to Russell and Karen got married Friday (February 1st, 2013). Russell presenting his poster at the Glycobiology Society Meeting. Srona presenting her poster at the ASBMB in San Diego.
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Directions
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Dr Zachara's Faculty Page. Dr Zachara's Faculty Page. Characterizing a novel cytoprotective pathway. Department of Biological Chemistry. The Johns Hopkins University School of Medicine. 725 N Wolfe Street. Baltimore, MD 21205-2185. We are located in building 19 in the attached. We are located at the East Baltimore Campus of the Johns Hopkins University. Marissa, Peter and Thiago. Whose review “. Stress-Induced O-GlcNAcylation, an Adaptive Process of Injured Cells” i. Austin has been awarded the 2016.
