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DNA Thermodynamics & Hybridization - IDT Biophysics

DNA Thermodynamics and Hybridization. Oligo Target ↔ Duplex. Mismatch, Dangling Ends. Native DNA: A, C, G, T. Locked nucleic acids: A, C, G, T. Software version 1.02.

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DNA Thermodynamics & Hybridization - IDT Biophysics | biophysics.idtdna.com Reviews
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DNA Thermodynamics and Hybridization. Oligo Target ↔ Duplex. Mismatch, Dangling Ends. Native DNA: A, C, G, T. Locked nucleic acids: A, C, G, T. Software version 1.02.
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1 DNA
2 duplex
3 oligomer
4 thermodynamic
5 melting temperature
6 spectrum
7 nucleic acid
8 stability
9 extinction coefficient
10 folding
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dna thermodynamics,uv spectrum,publications,idt publications,technical bulletins,tool help,sequence,bases,oligo conc,target conc,conc,dntps conc,base sequence symbols,inosine i,integrated dna technologies
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DNA Thermodynamics & Hybridization - IDT Biophysics | biophysics.idtdna.com Reviews

https://biophysics.idtdna.com

DNA Thermodynamics and Hybridization. Oligo Target ↔ Duplex. Mismatch, Dangling Ends. Native DNA: A, C, G, T. Locked nucleic acids: A, C, G, T. Software version 1.02.

INTERNAL PAGES

biophysics.idtdna.com biophysics.idtdna.com
1

IDT Biophysics Publications

http://biophysics.idtdna.com/IDTPublications.html

A bibliography of research papers published by the Biophysics group at IDT. Click on the reference and you will be redirected to the abstract. If available, you will be able to obtain a PDF download of the paper through the publisher site. Owczarzy R, You Y, Groth CL, and Tataurov AV (2011) Stability and mismatch discrimination of locked nucleic acid-DNA duplexes. You Y, Tataurov AV, and Owczarzy R (2011) Measuring thermodynamic details of DNA hybridization using fluorescence. Owczarzy R, You Y, Moreira ...

2

UV spectrum of oligonucleotides - IDT Biophysics

http://biophysics.idtdna.com/UVSpectrum.html

UV Spectrum of DNA. Software version 1.02.

3

Help - Hybridization Calculator - IDT Biophysics

http://biophysics.idtdna.com/HelpMelt.html

DNA Thermodynamics and Hybridization. How do I use the tool? Box is used to enter an oligonucleotide sequence. Sequences containing native DNA (A, T, C, G, I) and LNA nucleotides ( A, C, G, T) can be calculated. Box is used to enter the oligo concentration and has the upper limit of 10. 181;M (10 mM). Default value is 0.25 µM. Box is used to enter concentration of the target sequence that is complementary to the oligo. Concentrations up to 10. With DNA is different than under saturating conditions and pr...

4

Help - UV Spectrum Calculator - IDT Biophysics

http://biophysics.idtdna.com/HelpUV.html

UV spectrum of DNA calculator. Can I predict spectrum of RNA? No Only sequences containing standard DNA bases (A, T, C, and G) can be calculated. Has the algorithm been published? The extinction coefficient of DNA is calculated from the nearest-neighbor model at 260 nm. The parameters are reported in the following publications:. Fasman, G.D. (Ed.) (1975) Handbook of Biochemistry and Molecular Biology, Volume 1: Nucleic Acids, pp 589, 3rd edition, CRC Press. What is the percent error of the predictions?

5

IDT Technical Bulletins

http://biophysics.idtdna.com/TechBulletins.html

Here you can find current biophysical technical bulletins. Click on the link and a new window will open with a PDF copy of the bulletin. Calculation of Tm for Oligonucleotide Duplexes. Fluorescence and Fluorescence Applications. Fluorescence quenching by proximal G bases.

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Nucleic acid thermodynamics - Wikipedia, the free encyclopedia

https://en.m.wikipedia.org/wiki/Nucleic_acid_thermodynamics

Read in another language. Is the study of how temperature. Affects the nucleic acid structure. DsDNA). The melting temperature ( T. Is defined as the temperature at which half of the DNA strands are in the random coil. Or single-stranded (ssDNA) state. T. Depends on the length of the DNA molecule and its specific nucleotide. Sequence. DNA, when in a state where its two strands are dissociated (i.e., the dsDNA molecule exists as two independent strands), is referred to as having been denatured. Is used in...

owczarzy.net owczarzy.net

Melting temperature, Tm, of DNA oligonucleotide

http://www.owczarzy.net/tm.htm

DNA melting temperature - How to calculate it? I have developed on-line T. Calculations at Integrated DNA Technologies. Use our software tool. To estimate the T. Melting temperatures ( T. Of short DNA duplex oligomers ( 70 base pairs) are calculated using the following equation,. Is the ideal gas constant (1.9865 cal.mol. The concentrations (mol/L) of each strand are denoted C. The formula is valid if C. Is greater or equal to C. For any self-complementary strand, there is just one strand, so set C.

owczarzy.net owczarzy.net

Science of nucleic acids and proteins

http://www.owczarzy.net/scienceLinks.htm

Science of nucleic acids and proteins. Nano, quantum, thermodynamic values. NIST chemical and physical databases. PseudoViewer of RNA pseudoknots. American Society for Biochemistry and Molecular Biology. Gibbs Society of Biological Thermodynamics. DNA, RNA, PROTEIN STRUCTURE AND THERMODYNAMICS. OBRC-Database of bioinformatics databases and tools. NTDB-Thermodynamic database for nucleic acids. NDB - Nucleic acid database of 3D structures. European ribosomal RNA database. PPD - Protein pKa.

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DNA Thermodynamics & Hybridization - IDT Biophysics

DNA Thermodynamics and Hybridization. Oligo Target ↔ Duplex. Mismatch, Dangling Ends. Native DNA: A, C, G, T. Locked nucleic acids: A, C, G, T. Software version 1.02.

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